Subcellular Localization of H202 Production in Human Neutrophils Stimulated With Particles and an Effect of Cytochalasin-B on the Cells
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چکیده
The ultrastructural localization of H202 production in suspended polymorphonuclear leukocytes (PMN) stimulated with particles was studied using CeCI3 technique. PMN stimulated with opsonized zymosan or polystylene latex with or without lgG were incubated in 0.1 M Tris-maleate buffer with 1 mM CeCI, and 10 mM aminotriazole. Cells were then fixed and embedded in a resin for electron microscopy. The reaction product of cerium perhydroxide was observed on the phagosomal membranes and on the areas of the plasma membrane engulfing the particles. Catalase or ferricytochrome-c decreased the deposits. p-Benzoquinone (#{176}2 scavenger) inhibited the formation of
منابع مشابه
Subcellular localization of H2O2 production in human neutrophils stimulated with particles and an effect of cytochalasin-B on the cells.
The ultrastructural localization of H2O2 production in suspended polymorphonuclear leukocytes (PMN) stimulated with particles was studied using CeCl3 technique. PMN stimulated with opsonized zymosan or polystylene latex with or without IgG were incubated in 0.1 M Tris-maleate buffer with 1 mM CeCl3 and 10 mM aminotriazole. Cells were then fixed and embedded in a resin for electron microscopy. T...
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